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Twelve plant families containing 40 species (totally 51 cultivars) were inoculated with a China isolate of the tomato powdery mildew (Oidium neolycopersici) to determine its host range.There was no significant variation in susceptibility within species with the exception of Solanaceae, Leguminosae and Malvaceae in comparison with other studies.Macroscopically,infection was observed on some eggplant (Solanum melongena) cultivar, potato (S.tuberosum),some wild species of tomato(S.habrochaites, S.peruvianum, S.pimpinellifolium, S.pennellii and S.hirsutum)and as well as tobacco(Nicotiana.tabacum cv Wisconsin 38, N.tabacum cv.NC89 and N.Xanthii).Three species of soybean (Glycine max), black nightshade (S.nigrum),tobacco (N.tabacum cv Wisconsin 38 and N.tabacum cv.NC89) and Malope trifida were as susceptible to O.neolycopersicias tomato (S.lycopersicum cv Moneymaker), suggesting that these plants were the alternative hosts of tomato powdery mildew in China, which varied from other researches.