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Methods Quantitative real-time PCR was performed to determine miR-26b expression in obese rodent models, human obesity subjects and insulin-resistant adipocytes.We analysed the roles of miR-26b overexpression and inhibition on glucose uptake in adipocytes.Western blotting was used to detect the levels of protein molecules involved in the PI3K pathway.Bioinformatics and the Dual Luciferase Assay were used to identify the target gene of miR-26b.We assessed the regulatory roles of miR-26b on the PTEN/PI3K/AKT pathway and the relationship between miR26b and the metabolism of human obese subjects.