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Types2 diabetes in Balb/C mice is provoked by a high fat diet and is characterized by glucose intolerance and insulinresistance. Many researchers suggest that chronic exposure to phthalates such as DEHP produce effects consistent with diabetes andmay increase the risk of diabetes in obese patients. Our study was focalised to the characterization of glucose homeostasis effects ofBalb/C mice exposed to high concentration of DEHP and fed fat diet. Mice were fed high fat and normal diet while exposed to 500mg and 1000 mg/kg b.w of DEHP until ten weeks; other two groups were fed normal and fat diet with saline. After 4 weeks ofexposure, glucose and body weight were monitored and those exposed to DEHP and fed fat diet are divided into two groups, onereceived vitamin E supplementation. After ten weeks of exposure, blood glucose and insulin level were checked after overnightfasting and HOMA-IR was calculated. All results were analyzed using Origin V6.10.52 software. In our study, normal diet and fatdiet exposed mice accumulate more weight and glucose tolerance was higher in the 1000 mg/kg b.w DEHP exposed mice fed fat dietwhile the insulin level decrease in these groups of mice. HOMA-IR level was higher in high DEHP exposed mice fed fat diet andthose three indicators vary in dose and diet-dependent manner. We noted that there is a low effect of vitamin E for suppressing DEHPand fat effects but the difference was not significant.