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Objectives Silencing Geminin gene selectively by using RNA interference and investigate the mechanism of the Geminin gene silence regulating VSMCs proliferation.Methods VSMCs proliferation were determined by [3H]-thymidine incorporation, 5-ethynyl-2-deoxyuridine (EdU) incorporation and flow cytometry;mRNA and Protein expression of Geminin and Cdt1 were determined by real time-PCR, westernblotting, and immunohistochemistry;the interrelationships of Geminin and Cdt1 were examed by immunofluorescence and co-immunoprecipitation (Co-IP).