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Objective: To examine whether autophagy inhibitor chloroquine (CQ) can sensitize the esophageal cancer cell line TE-1 to IR.Methods: The effect of CQon cell viability of TE-1 cells was determined using MTT.The IC10 and IC50 values of CQwere calculated using the software, and the concentration of IC10 was chosen for the following experiments.Two groups of different doses of IR alone were designed, along with different dosing times of CQcombined with IR.After treatment with IR,expressions of autophagy-related proteins LC3 and Beclin-1 were detected by Western blot at different time points.The formation of acidic vesicular organelles (AVOs) was determined by fluorescence staining with double staining of Lyso-Tracker Red DND-99 and Hoechst 33258.Early-stage apoptosis at 6 h and24 h after different treatments were measured by Annexin-Ⅴ/PIstaining through flow cytometry.Clonogenic survival of TE-1cells upon treatment with addition of CQbefore or after exposure to IR was examined by clonogenic forming assay.The radiobiology parameters were estimated from the curve fitted by the single-hit multi target model [SF =l-(-e-D/D0)N] and linearquadratic model [SF =e^(-αD-βD2)].