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  5. Expression analysis of a transformer gene in Daphnia pulex after RNAi

Expression analysis of a transformer gene in Daphnia pulex after RNAi

来源 :第十届世界华人虾蟹养殖研讨会暨2016年中国河蟹产业发展高峰论坛 | 被引量 : 0次 | 上传用户：xiaozhenying
【摘 要】
：
In order to explore the importance of the transformer (tra) gene in reproductive mode switching in Daphnia pulex,we studied the effect of silencing of this gene using RNA interference (RNAi).We obtain
【作 者】
：
Chunyang GUO Xiao CAO Yibo ZHANG Danli WANG Yunlong ZHAO 
【机 构】
：
School of Marine Science, Ningbo University, Ningbo China, 315211
【出 处】
：
第十届世界华人虾蟹养殖研讨会暨2016年中国河蟹产业发展高峰论坛
【发表日期】
：
2016年8期
【关键词】
：
RNAi RT-PCR Western blotting reproductive transformation parthognesis cladoceran 
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　　In order to explore the importance of the transformer (tra) gene in reproductive mode switching in Daphnia pulex,we studied the effect of silencing of this gene using RNA interference (RNAi).We obtained Dptra dsRNA by constructing and using a dsRNA expression vector and transcription method in vitro.D.pulex individuals in different reproductive modes were treated by soaking in a solution of Dptra dsRNA.We then assayed the expression of the endogenous Dptra mRNA after RNAi treatment using RT-PCR and obtained the suppression ratio.
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