Effect of FGF-21 on triglyceride of nonalcoholic fatty liver disease model in vivo and vitro

• 作 者: Lizhen chen Yongning Xin Jian Wang Yang Liu Xiangjun Jiang Shiying Xuan
• 机 构: Department of Gastroenterology,Qingdao Municipal Hospital,School of Medicine,Qingdao University,Qing
• 出 处: 中华医学会第十七次全国病毒性肝炎及肝病学术会议暨2015年中华医学会感染病学分会年会、中华医学会肝病学分会年会
• 发表日期: 2015年3期
• 关键词: Fatty liver nonalcoholic fibroblast growth factor-21 triglycerides

　　Objective: To investigate the effect of fibroblast growth factor-21(FGF-21)on triglyceride(TG)of nonalcoholic fatty liver disease(NAFLD)in vivo and vitro.Methods:(1)Free fatty acids(FFA)mixture was used for model building of steatosis in vitro.Then LO2 cells were treated with FGF-21 or fenofibrate.After 24 hours,Oil red O staining was performed to observe the degree of steatosis and intracellular TG contents were detected.The mRNA and protein of SREBP-1c were examined by RT-PCR and Western blot.(2)High fat diet(HFD)was used to establish the animal model of NAFLD.HFD mice were treated with FGF-21 or fenofibrate for 8 weeks.The liver samples were stained with HE.Meanwhile,levels of serum TG,ALT,AST and TG contents of liver samples were measured.