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This paper described a new assay for real-time investigation on the interaction between protein and photosensitizer of photodynamic therapy (PDT) using a quartz crystal microbalance (QCM) biosensor. The new generation photosensitizer, meso-tetrakis(4-hydroxyphenyl)porphyrin (p-THPP), was immobilized on the gold electrode of the QCM chip by combining sol-gel and self-assembly method. The binding interactions of the immobilized p-THPP with various proteins in solution were monitored as the frequency variations of the modified sensor. Such a rapid screen analysis of interactions showed that p-THPP-immobilized sensor was sensitive and specific interaction only with hemoglobin (Hb)(Fig.1).