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Gambogic acid (GA), a natural compound from gamboge resin, has been introduced as a promising antitumor drug contributing to its broad spectrum of antitumor activity.However,the poor aqueous solubility and short half-life hinder its clinical application.Pluronic F68 (F68) is a well-known amphiphilic block copolymer consisting of hydrophobic propylene oxide units and hydrophilic ethylene oxide.Although F68 has an amphiphilic structure, its short propylene oxide segment limits its dilution stability and drug loading capacity.To overcome this limitation, we modified F68 by conjugating linoleic acid, a hydrophobic fatty acid, to prolong the length of ethylene oxide segment and thus improve the stability of F68 micelles.This novel F68-1inoleic acid (F68-LA) conjugate was successfully synthesized and was used to load GA to improve its anti-cancer effects.GA-loaded F68-LA micelles were stable for 6 days, with a mean diameter of 159.3 nm and zeta potential of-23.2 mV.The entrapment efficiency of GA in F68-LA micelles was as high as 92.0%.Furthermore,F68-LA/GA micelles exhibited enhanced cytotoxic activity and proapoptotic effect against human ovarian cancer A2780 cells, compared with free GA.Our results showed that F68-LA/GA micelles might be a promising cancer targeted drug delivery system in ovarian cancer therapy.