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Zinc is one of the demand essential elements and its deficiency is a critical nutritional and health problem in the world.The organic forms of trace element are considered more bioavailable than the inorganic ones.It is a very important attempt and choice to obtain bound organic zinc by microorganism transformation.Therefore, twenty-two strains which tolerated high zinc were screened and isolated from natural environment, and only one strain No.LZ-1108 grew well with 10,000μtg/mL zinc(Ⅱ).According to morphological, 18S rDNA sequence analysis, biophysical and biochemical characteristics, No.LZ-1108 is identified as Fusarium oxysporum.The cells zinc content in No.LZ-1108 was 6.7mg/g dry cell.The bioavailability in rats confirmed that No.LZ-1108 had higher absorption efficiency than gluconate zinc.Our study would bring new ideas for finding new zinc supplements from natural environment.