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Histidine-rich protein 2(HRP 2) is the major protein released into the blood of an infected person by plasmodium falciparum,the parasite that causes the most severe form of human malaria.The HRP 2 gene has two exons separated by an intron.In this study,we expressed and purified products of exon Ⅱ from E.coli to evalu ate its immunogenicity potential for use in developing monoclonal antibodies against the native form.This recom binant protein was use to immunize mice for the development of monoclonal antibodies.Two monoclonal antibod ies(1AS and 1C10) with very high affinity,specificity and sensitivity for both native and recombinant HRP 2 were selected.The Ka value of mAb 1A5 and 1C10 is 7.15×10-7 and 4.91×10-7 respectively.These results suggest that partially expressed HRP 2 in the exon II region contain immunogenic sites similar to those in the native antigen and can be used for the development of monoclonal antibodies suitable for the diagnosis of Plasmodium falci parum infection in endemic communities.