MLN4924 inhibits neointima hyperplasia and promotes apoptosis of vascular smooth muscle cells throug

来源 :Ready for 2018- the 4th China International Forum on Hyperte | 被引量 : 0次 | 上传用户:shenjin62
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Objective:To explore the contribution of the neddylation in neointima formation after vascular injury and study the mechanisms.Methods:Twelve C57 mice undergoing femoral artery injury, then subcutaneous injections MLN4924 (the inhibitor of NAE) or not and tissue sections were used to evaluate the impact of MLN4924 on neointima formation and vascular remodeling.Immunofluorescence was used to measure cell proliferation.TUNEL staining was used to measure the apoptosis.Flowcytometry was used to detect cycle arrest.RT-QPCR was used to measure gene expression and Western blotting was used to measure protein expression, siRNA were used to study the role of p62 and p53 in the apoptosis and the relationship between p62 and p53 in vascular smooth muscle cells (VSMCs).
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