【摘 要】
：
黄曲霉毒素B1(Aflatoxin B1,AFB1)和脱氧雪腐镰刀菌烯醇(deoxynivalenol,DON)是饲料和食品中常见的霉菌毒素,严重危害动物和人类的健康.在本试验中,以锦鲤原代肝细胞为研究对
【机 构】
：
南京农业大学动物医学院,南京210095苏州市吴江区农业委员会,吴江215200
【出 处】
：
中国畜牧兽医学会动物毒物学分会第十三次学术研讨会
论文部分内容阅读
黄曲霉毒素B1(Aflatoxin B1,AFB1)和脱氧雪腐镰刀菌烯醇(deoxynivalenol,DON)是饲料和食品中常见的霉菌毒素,严重危害动物和人类的健康.在本试验中,以锦鲤原代肝细胞为研究对象,研究AFB1和DON单独和联合对其影响.结果表明:AFB1和DON联合组(0.01μg/mL AFB1和0.25μg/mL DON; 0.02μg/mL AFB1和0.25μg/mLDON; 0.02 AFB1和and 0.5 u g/mL DON)的毒性明显高于单独毒素组(p＜0.05).当毒素与细胞作用4h后,细胞上清液中AST,ALT和LDH的活性也高于相应对照组.光黄曲霉毒素B1(aflatoxin B1,AFB1)AFB1为已知的致癌物,同时还具有致突变作用和致DNA损伤作用.研究发现AFB1对人类染色体具有致畸变作用(余新生等,1981).流行病学研究表明,AFB1与人类肿瘤,如原发性肝癌有密切的关系,是原发性肝癌的病因之一(李斌等,2001).AFB1可直接作用于核酸合成酶而抑制信使核糖核酸(m-RNA)合成作用,并进一步抑制DNA合成,而且对DNA合成所依赖的RNA学显微镜下发现两种毒素均能在短时间内引起细胞的大量死亡,各试验组均与对照组之间差异显著.在电镜下,和对照组相比,低浓度联合毒性组和DON组均能发现数量不等的凋亡细胞,而低浓度的AFB1组则仅见个别凋亡的肝细胞.通过研究表明两种毒素的联合毒性应该具有协同作用,但其机理还有待进一步研究.
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