【摘 要】
：
High-throughput RNA sequencing was performed for comprehendsively analyzing the transcriptome of thegeese.A total of 28,803,759 by of raw sequence data were generated,of which 28,730,361bp remained af
【出 处】
：
第七次全国动物生物技术学术研讨会暨新疆畜牧科学院第六次学术年会
论文部分内容阅读
High-throughput RNA sequencing was performed for comprehendsively analyzing the transcriptome of thegeese.A total of 28,803,759 by of raw sequence data were generated,of which 28,730,361bp remained afteradaptor trimming (99％ of sequenced bases).After removal of adaptor sequences,117,279 reads were obtained,with an average length of 244 bases.Assembly ofall the reads from both 454 and illumine platforms,formed56,839 contigs,with 12,995 readsremaining as singletons,Ranging from 38 to 28206 by in size,with an averagesize of 2584 by and an N50 of 4624.The assembly produced a substantial number of large contigs:35,545 (62.5%)were longer thanlkb,of which 8850 15.6%)were longer than Skb.The sequencing depth was 85 X on average.Weperformed comprehensive function annotations on unigenes including protein sequence similarity,GO termclassification,and KEGG pathway enrichment.Gene ontology (GO) analysis showed that approximately 63% ofthe contigs had annotation information,among the 36,756 annotated sequences in Nr database,24,299(66.1%)sequences were assigned with one or more GO terms.There were 19,757(81.3%)isotigs for biological processes,10,643(43.4%)isotigs for cellular component,22818 (93.9%) isotigs for molecular function.The result of KEGGpathway mapping 8,737 sequences had the pathway annotation,and took part in477 pathways.In addition,different expression genes in ovarian tissue between different states of Sichuan White geese were detected in thecurrent study.
其他文献
Sertoli-Sertoli-germ cell tight and adherent junctions may play an essential role in spermatogonial stem cells (SSCs) to differentiate spermatocytes and functional haploid spermatozoa.In this study, w
本文描述了优良猪种的持续选育改良的意义。分析了基因组学的发展为猪育种提供了技术保障。阐述了基因组选择和大量因果突变位点在育种实践中的应用虽将成为未来主流趋势，但业存在很多难点，包括基因组选择需要构建大群体的参考家系，特定种群的遗传效应难以代表所有商业品种(系)的遗传背景;对于基因与环境互作效应大、遗传力低的经济性状的选育，不管通过基因组选择还是搜寻主效基因的策略都有一定的难度等问题。
本试验建立并优化了少量细胞全基因组mRNA测序程序，对小鼠的体内胚、猪的体内胚以及猪体细胞核移植胚进行了全基因水平转录组检测和分析，揭示了猪附植前胚胎发育中存在的特异的基因表达模式和调控网络。通过对两个连续阶段的分析，发现合子基因组激活在猪的体内胚中发生在四细胞阶段，小鼠体内胚发生在二细胞阶段，然而猪的体细胞克隆胚延迟到了八细胞阶段。这表明核移植操作影响了克隆胚的转录组模式。
利用羊SNPSOK芯片分析技术、高通量测序技术和绵羊全基因组遗传信息，结合分子生物学研究手段和方法，从全基因组水平了解新疆绵羊主要品种的起源进化、评估不同地方绵羊品种的遗传结构和遗传多样性、研究绵羊体格大小和毛色等性状形成过程中的分子机制等方面入手，开展了与绵羊经济性状相关的功能基因的研究，为有效保护新疆绵羊品种遗传资源、充分发挥其在绵羊品种改良和培育的遗传潜力和优势奠定基础。
从miRNAs调控乳腺脂肪酸代谢的角度出发，通过Solexa测序技术获得西农萨能奶山羊乳腺组织miRNAs表达谱，并分析了山羊miRNAs序列特征。选择前30个高丰度miRNAs，检测其在乳腺组织不同阶段的表达水平，得到17个差异表达miRNAs。利用GO和KEGG软件预测miRNAs的功能，从差异表达miRNAs中筛选获得可能调控脂肪酸代谢的miR-27a/103/200a。利用腺病毒在山羊乳腺
Introduction：In the long period of natural selection and artificial selection,people began to plant alfalfa for cattle feed on theGuanzhong Plain,the main production area of Qinchuan beef cattle.This
本实验采用环介导等温扩增方法，通过在线软件设计了2对引物，并优化了反应温度、时间、酶的用量等条件，建立了检测人乳铁蛋白基因和人溶菌酶基因的扩增体系，检出限为每微升10拷贝，是普通PCR的10倍。并对41个转人乳铁蛋白基因山羊样品和31个转人溶菌酶山羊进行了盲检检测，检测结果与预期一致。同时本实验室正在研发用于检测转人乳铁蛋白基因、人乳清蛋白基因、人溶菌酶基因转基因牛的纳米金基因芯片。根据这三个基因
Bcllla是一个C2H2锌指转录因。前期研究表明Bcllla对维持胚肝中淋巴细胞的正常发育是必需的;Bcllla敲除的胚肝缺失B淋巴细胞和产生不正常T淋巴细胞。由于Bcllla敲除的小鼠新生胎儿致死，为研究Bcllla在成年动物淋巴细胞生成中的作用及机制，本文构建了Bcllla的条件性敲除小鼠。本研究证明了Bcllla是淋巴细胞发育的重要调控因子，B淋巴细胞和CLPs的存活和增殖依赖于Bclll