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Fruit-bagging has been used as a pest management method to control fruit moths on several continents.With the quickly increasing apple planting area in China,fruit-bagging was becoming the most important fruit borer control strategy in many apple growing area.However,nothing is known about how this physical control tactic affects the population genetics of the oriental fruit moth Grapholita(=Cydia)molesta(Busck).We used five polymorphic microsatellite loci and two mitochondrial gene sequences to investigate the genetic diversity and genetic structure of G.molesta populations from three fruit-bagged and two fruit-unbagged apple orchards in the respective five regions of a major fruit growing area of China.Adult moths were collected throughout the season from apple.We found high numbers of microsatellite alleles and mitochondrial DNA haplotypes.Populations from fruit-bagged and unbagged orchards were genetically significantly differentiated,indicating an effect of the pest management method applied.Genetic structures of the populations under a given management regime changed in the course of the season in a characteristic manner each,and within the same regime,patterns were always similar at a given sampling time.There was no significant isolation by geographical distance.Population genetics from the two orchard types did not resemble one another at the beginning,but became similar by the end of the season.Pest management methods applied can have an important impact on the genetic diversity and genetic structure of targeted pest insect populations.