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Objectives: We aimed to identify metastasis-related biomarkers in human ovarian cancer cell lines and serum.Methods: We isolated total proteins from cell lysis solutions and culture supematants from two human ovarian cancer cell lines and used SELDI-TOF-MS to detect the differential expression of the proteins in the two cell lines.The proteomic spectra were generated using weak cation exchange chips.The biomarkers were validated by analyzing serum proteins or peptides in ovarian cancer patients, relapsed ovarian cancer patients, patients with benign ovarian tumors, and healthy people.