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In this paper, concentrations of S2O32-and SO32-were measured at the following gold leaching systems, including thiosulfate system, polysulfide system and the modified lime sulfur synthetic solution (ML) system in the process of chemical reaction.The interactions among S2O32-, SO32-and S2-were discussed.The behavior mechanism of sulfur-bearing reagents was proposed to describe the process reactions and their Gibbs free energy.The proper quantity oxygen and SO32-reduced decomposition of S2O32-and reacted with sulfur derived from the decomposition of Sx2-.So SO32-ions have action to stabilize sulfur-bearing system and were favorable to leach gold.