【摘 要】
：
This study is to observe the protecting effects of melatonin on the hemoglobin re-releasedfrom hydrogen peroxide (H2O2) damaged red blood cells (RBCs).Firstly, RBCs weretreated with 0.25％, 0.5％, 1％, 2
【机 构】
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Department of Biochemistry and Biology, Baotou Medical College
【出 处】
：
中国生物化学与分子生物学会第十一次会员代表大会暨2014年全国学术会议
论文部分内容阅读
This study is to observe the protecting effects of melatonin on the hemoglobin re-releasedfrom hydrogen peroxide (H2O2) damaged red blood cells (RBCs).Firstly, RBCs weretreated with 0.25％, 0.5％, 1％, 2％ and 4％ H2O2 for 1 hour respectively, then the concentrationsof Malondialdehyde (MDA) in each samples supernatant were detected withMDA assay kit (TBA method), and the amount of hemoglobin re-released from the oxidationinjured RBCs was observed by performing agarosestarch mixed gel electrophoresis.After finding the proper concentration that could cause the re-released hemoglobinchange significantly, the time-dependant (20min, 40min, 60min, 80min, 100min, 120min,150min, 180min and 240min) effects of H2O2 on the re-released hemoglobin were observedat this concentration.
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