【摘 要】
：
Cytoplasmic male sterility (CMS) is a widespread phenomenon in higher plants, and several studies have established that this maternally inherited defect is often associated with a mitochondrial mutant
【机 构】
：
National Key Laboratory of Crop Genetic Improvement,Huazhong Agricultural University,Wuhan 430070,Ch
【出 处】
：
中国作物学会油料作物专业委员会第七次会员代表大会暨学术年会
论文部分内容阅读
Cytoplasmic male sterility (CMS) is a widespread phenomenon in higher plants, and several studies have established that this maternally inherited defect is often associated with a mitochondrial mutant.Approximately 10 chimeric genes have been identified as being associated with corresponding CMS systems in the family Brassicaceae, but there is little direct evidence that these genes cause male sterility.In this study, a novel chimeric gene (named orf288) was found to be located downstream of the atp6 gene and co-transcribed with this gene in the hau CMS sterile line.Western blotting analysis showed that this predicted open reading frame (ORF) was translated in the mitochondria of male-sterile plants.Furthermore, the growth of Escherichia coli was significantly repressed in the presence of ORF288, which indicated that this protein is toxic to the E.coli host cells.To confirm further the function of orf288 in male sterility, the gene was fused to a mitochondrial-targeting pre-sequence under the control of the Arabidopsis APETALA3 promoter and introduced into Arabidopsis thaliana.Almost 80% of transgenic plants with orf288 failed to develop anthers.It was also found that the independent expression of orf288 caused male sterility in transgenic plants, even without the transit pre-sequence.Furthermore, transient expression of orf288 and green fluorescent protein (GFP) as a fused protein in A.thaliana protoplasts showed that ORF288 was able to anchor to mitochondria even without the external mitochondrial-targeting peptide.These observations provide important evidence that orf288 is responsible for the male sterility of hau CMS in Brassica juncea.
其他文献
Meiotic nondisjunction during microsporegenesis can lead to aneuploid gametes formation and reduced pollen fertility in plants.This paper reports the prevalence of meiosis Ⅰ nondisjunction in a resynt
Yellow-seeded Brassica napus was for the first time developed from interspecific crosses using yellow-seeded B.juncea (AABB), yellow-seeded B.oleracea (CC), and black-seeded artificial B.napus (AACC).
Bleomycin, a glycopeptide antibiotic produced by the bacterium Streptomyces verticillus, has been demonstrated to be an effective mutagen in Arabidopsis thaliana.The objective of the present study was
7365AB, a recessive genetic male sterility (RGMS) system, is controlled by BnMs3 in Brassica napus which encodes a Tic40 protein required for tapetum development.However, the role of BnMs3 in rapeseed
Here, we describe the characteristics of a Brassica napus male sterile mutant 7365A with loss of the BnMs3 gene, which exhibits abnormal enlargement of the tapetal cells during meiosis.Later in develo
S45A, a double recessive mutant at both BnMs1 and BnMs2 loci in Brassica napus, produces no pollen in mature anthers and no seeds by self fertilization.The BnMs1 and BnMs2 genes, which have redundant
Sclerotinia stem rot, caused by fungus Sclerotinia sclerotiorum, is one of the most devastating diseases of rapeseed (Brassica napus L.) in China.The two major factors in limiting developing disease r
Yellow seed color, which results from a thinner seed coat, is associated with improved feed quality of rapeseed meal and increased oil and protein contents.However, this trait follows various genetic
Both the pollination control system and genetic distance are major factors in the utilization of crop heterosis.The recessive genic male sterile line (RGMS), 7-7365A (Bnms3ms3ms4ms4), has been widely
Male sterility plays an important role component of heterosis utilization in Brassica napus L.The B.napus line,7-7365ABC, is a recessive epistatic genic male sterile (REGMS) three-line system.The phen