A novel Rolling circle amplification and DNA origami based DNA belts involved signal amplification a

• 作 者: JuanYan[1]ChongyaHu[2]PingWang[1]RuiLiu[1]XiaoleiZuo[3]ShipingSong[3]ChunhaiFan[3]DannongHe[4]
• 机 构: National Engineering Research Center for Nanotechnology,Shanghai 200241,China
• 出 处: 中国微米纳米技术学会纳米科学技术分会第三届年会暨2014全国纳米生物与医学学术会议
• 发表日期: 2014年期

　　Prostate-specific antigen(PSA)is one of the most important biomarkers for the early diagnosis and prognosis of prostate cancer.Although many efforts has been made to achieve significant progress for the detection of PSA,challenges including relative low sensitivity,complicated operation,sophisticated instruments and high-cost remain unsolved.Here,we have developed a strategy combining rolling circle amplification(RCA)-based DNA belts and magnetic beads based enzyme-linked immunosorbent assay(ELISA)for the highly sensitive and specific detection of PSA.