【摘 要】
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We conducted a large-scale survey on the existence and expression status of porcine endogenous retrovirus (PERV)in seven breeds of Chinese miniature pigs. Genotyping of PERV was examined by PCR using
【机 构】
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Department of Blood Biopharmaceuticals and Biotechnology,Beijing Institute of Transfusion Medicine,
【出 处】
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2011东莞第二届国际小型猪学术论坛暨大型实验动物生物医药研究应用研讨会
论文部分内容阅读
We conducted a large-scale survey on the existence and expression status of porcine endogenous retrovirus (PERV)in seven breeds of Chinese miniature pigs. Genotyping of PERV was examined by PCR using type-specific primers according to theenv genotyping method. The presence and expression status of viral gag, pol and env genes were further analyzed in Wuzhishan pigs(WZSP) and Bama minipigs (BMP). The results showed that PERV existed in all 348 genomic DNA samples. The genotypedistribution was: subtype A--74.43%, subtype B--95.40%, subtype C--30.46%. No expression of subtype C was found in WZSP andBMP This research obtained an adequate level of information on the molecular epidemiology of PERV in China. The resultsindicated that its possible to monitor pig herds for individuals with the lowest PERV prevalence, especially lacking PERV-C.
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