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目的建立Wistar大鼠C6胶质瘤模型,采用MRI、CT灌注(CT perfusion,CTP)成像观测肿瘤体积的动态变化,探讨大鼠C6脑胶质瘤的生长规律。方法成年Wistar大鼠40只,采用立体定向仪进行C6细胞脑内接种,建立大鼠C6脑胶质瘤模型。每次随机抽取10只接种鼠分别对应于5~9 d、10~14 d、15~19 d 3个时间段行CTP、MRI及病理学检查,观测大鼠C6脑胶质瘤的生长规律,并评价影像学与病理学结果之间的相关性。结果15~19 d时间段CTP、MRI观测结果分别与病理学结果比较均有显著性差异(t=3.131,P<0.01;t=2.566,P<0.05),但CTP与MRI观测结果差异无统计学意义(P>0.05)。5~9 d和10~14 d时间段3种技术观测结果比较差异均无统计学意义(P>0.05)。Pearson线性相关分析表明CTP、MRI与病理学三者测量结果间均有显著正相关,病理学与CTP、病理学与MRI、CTP与MRI的r=0.998,0.998,1.000(P<0.01)。回归分析表明,肿瘤体积随时间呈指数规律增长(rpatho=0.990,rCTP=0.987,rMRI=0.990,P<0.01)。结论影像学活体观测的准确性高,更真实反映肿瘤的实际大小,适于对肿瘤动物模型的生长观测和试验性治疗的疗效评价。
Objective To establish a rat model of C6 glioma in Wistar rats. The dynamic changes of tumor volume were observed by MRI and CT perfusion (CTP) imaging to investigate the growth pattern of C6 glioma in rats. Methods Forty adult Wistar rats were inoculated intracerebrally with C6 cells by stereotactic instrument to establish rat C6 glioma model. Each time randomly selected 10 inoculated rats corresponding to 5 ~ 9 d, 10 ~ 14 d, 15 ~ 19 d three time periods underwent CTP, MRI and pathological examination of rat C6 glioma growth pattern, And evaluate the correlation between imaging and pathology results. Results The results of CTP and MRI from 15 to 19 days were significantly different from the pathological findings (t = 3.131, P <0.01; t = 2.566, P <0.05) Significance (P> 0.05). There was no significant difference between the three techniques in 5 ~ 9 days and 10 ~ 14 days (P> 0.05). Pearson linear correlation analysis showed that there was a significant positive correlation between CTP, MRI and pathology. Pathology and CTP, pathology and MRI, CTP and MRI r = 0.998,0.998,1.000 (P <0.01). Regression analysis showed that tumor volume increased exponentially with time (rpatho = 0.990, rCTP = 0.987, rMRI = 0.990, P <0.01). Conclusion The imaging accuracy of living observation is high, which reflects the actual size of tumor more accurately. It is suitable for evaluating the growth of experimental animal model and experimental treatment.