论文部分内容阅读
Objective To detect the expression of chemokine receptor CCR5 in human invasive ductal breast carcinoma and study the chemotaxis and invasion of breast cancer cells facilitated by chemokine receptor 5 (CCR5) and its ligand and explore the role of CCR5 in breast cancer.Methods Sixty-five samples of invasive ductal breast carcinoma and 30 samples of breast fibroadenoma specimens were analyzed separately by immunohistochemical staining for CCR5 expression.MIP-1 α,MIP-1 β and RANTES concentrations were measured by double-antibody sandwich ELISA in the sera of patients.Reverse transcription-polymerase chain reaction(RT-PCR) and immunohistochemical staining were used to detect CCR5 mRNA and protein expression in breast cancer cells MCF-7,the chemotaxis and invasion of MCF-7 were performed by Boyden chambers treated with regulated on activation normal T-cell expressed and secreted(RANTES),which is a ligand of CCR5.Results The expression of CCR5 was detected in human invasive ductal breast carcinoma (the positive rate was 64.6%)and was not detected in breast fibroadenoma.RANTES concentrations in the sera of breast carcinoma patients increased significantly compared with those in breast fibroadenoma patients (P<0.01) while there was no difference of MIP-1α and MIP-1β.MCF-7 expressed CCR5 mRNA and protein.RANTES-mediated chemotaxis and invasion was checked obviously in the breast cancer cells.The number of cells migrating and penetrating to the opposite side of polycarbonate membrane was significantly higher than that of negative control group.Both chemotaxis and invasion of RANTES-mediated were blocked evidently by anti-CCR5 neutralizing antibody.Conclusion CCR5 was expressed in human invasive ductal breast carcinoma and breast cancer cells MCF-7,and CCR5 facilitated chemotaxis and invasion of MCF-7.This indicates CCR5 and its ligand RANTES may play an important role in the pathogenesis of human breast cancer.