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Cr(Ⅲ) is often regarded as a trace essential micronutrient and can be found in many dietary supplements due to its participation in blood glucose regulation.High concentrations Cr(Ⅲ) exposure however,has been shown to have toxic effects.Herein,scanning electrochemical microscopy(SECM) depth scan imaging is employed to monitor the membrane permeability of single live human bladder cancer(T24) cells following 1 hr incubations with Cr(Ⅲ) stimuli.