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Ginkgolides are key pharmaceutical components in Ginkgo biloba.According to the cDNA sequence of MECPs and MECT gene to design primer, and we obtained the two genes promoter from Ginkgo genomic DNA using genome walking method.In this study, the promoters of MECPs and MECT, with 744 bp and 982 bp in length.The ciselements of GbMECPs and GbMECT promoters were predicted and analyzed by the plant cis-acting regulatory element (CARE) database.We found some major cis-element in the sequence of GbMECT and GbMECPs promoters.The GbMECPs promoter has six TATA box, eight CAAT box;and the GbMECT has five TATA box, seven CAAT boxes.Furthermore, some cis-elements in the promoters of GbMECPs and GbMECT promoter,including hormone, light-regulated elements, UB-B induced-elements and stress-related dehydration-responsive.The qRT-PCR showed that the MECPs gene are mainly involved in responses to CCC and UV-B, and MECTs are mainly responses to wounding treatment,these results also showed the expression model were in line with the cis-element.During the annual growth cycle, the GbMECPs were significantly correlated with sesterpene accumulation in leaves.A fitted quadratic curve showed the best model for relating GbMECPs with sesterpene in leaves.These results will help us to understand the transcriptional regulatory mechanisms involved in key gene expression and ginkgolide accumulation in G.biloba.