Overexpression of Brassica napus LPAT5 increased oil content in Arabidopsis thaliana seeds

• 作 者: YIN Yongtai CHEN Kang ZHANG Kai FU Chunhua ZHANG Chunyu LONG Yan YU Longjiang LI Maoteng
• 机 构: College of Life Science and Technology,Huazhong University of Science and Technology,PR China;Key La
• 出 处: 2016年作物学全国博士生学术论坛
• 发表日期: 2016年5期
• 关键词: Brassica napus BnLPAT5 Oil content fatty acid

　　Objectives:To investigate the role of lysophosphatidic acid acyltransferase 5 (LPAT5)acted in the oil accumulation progressin seed.Methods:RT-PCR was performed to cloneBnLPAT5 gene in B.napusdeveloping seeds which was collected 40 days after flowering.Real-time PCR was performed to investigate the expression of BnLPAT5 gene in various organs.Subcellular location detection was performed in N.Tabacum mesophyll cell and Arabidopsis mesophyll protoplastssubsequently.Toevaluate the role of BnLPAT5 acted in the oil accumulation progress,a binary plant expression vector was constructed and transformed into Arabidopsis.Theseed oil contents and fatty acid composition of T3 generation were detected by GC-MS.