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Europium can form a stable chelate with pyridine - 2,4,6 - tricarboxylic acid(PTA) having a ratio of 1:1 at a aqueous medium. The system, having the maximum excitation and emission wavelengths at 311. 0nm and 616.3nm, separately, shows a constant fluorescence intensity in the pH range of 10.0~12. 0. The method has high selectivity and sensitivity as well as the low effect of foreign ions. The optimum determinate conditions were obtained. The fluorescence intensity is a linear function of Eu3+ concentration in the range of 0.0029~ 1.520 μm/mL and the limit of detection is 2.90 ng/mL.