Highly efficient extraction of biological samples with mesoporous adsorbents for protein and peptide

• 作 者: HanfaZou;HongqiangQin;FangjunWang;RenanWu;
• 机 构: Key Laboratory of Separation Science for Analytical Chemistry,National Chromatographic R&A Center Da
• 出 处: 第八届全国微全分析系统学术会议、第三届全国微纳尺度生物分离分析学术会议暨第五届国际微化学与微系统学术会议
• 发表日期: 2013年期

　　Sample preparation has been playing an important role in the analysis of complex samples.Mesoporous materials as the promising adsorbents have gained increasing research interest in sample preparations due to their desirable characteristics of high surface area,large pore volume,tunable mesoporous channels with well defined pore-size distribution,controllable wall composition,as well as modifiable surface properties.The recent advances of mesoporous materials in sample preparation with emphases on the size selective enrichment of peptides/proteins,specific capture of post-translational peptides/proteins and enzymatic reactor for protein digestion will be presented.Furthermore,the target of cellular nucleus by nanomaterials for in-vivo extraction of nucleic acids associated proteins (NAaP) was developed,and proteomic profiling of the extracted proteins was performed.Such a strategy makes it possible for tracing of thousands of NAaP changing in living cells.