Andrographolide inhibits intracellular Ca2+release and caveolin-1 expression in myocardial microvasc

• 作 者: BoFeng[1]QianZhang[2]YingGao[3]XinWang[2]XiangwanSun[2]YunWang[2]XiangMu[2]HongDong[2]
• 机 构: Beijing Key Laboratory of Traditional Chinese Veterinary Medicine,Beijing University of Agriculture,
• 出 处: 第七届中国畜牧科技论坛
• 发表日期: 2016年期

　　The aim of this study was to investigate the effect of Andrographolide(ANDRO)on intracellular Ca2+release and the expression of caveolin-1(CAV1)in rat microvascular endothelial cells(RMMECs)and the underlying mechanism.We observed that ANDRO significantly inhibited extracellular ATP(eATP)-induced Ca2+release in primary RMMECs.In addition,ANDRO also exhibited inhibitory activity both on the mRNA and protein expression of phospholipase Cδ3(PLCδ3)and CAV1.We conclude that ANDRO reduced the expression of CAV1 and eATP-induced transient calcium release at least partially by decreasing the expression of PLCδ3.Our findings provide some insights into the mechanisms by which ANDRO can alter molecules involved in atherosclerosis(As).