论文部分内容阅读
目的通过观察脑缺血再灌注损伤后,大黄酚脂质体对脑源性神经营养因子(BDNF)和胶质酸性纤维蛋白(GFAP)表达的影响,探讨大黄酚脂质体对脑缺血再灌注损伤小鼠海马的保护作用及其机制。方法采用暂时性阻断颈总动脉的方法建立小鼠脑缺血再灌注损伤模型,腹腔注射大黄酚脂质体10.0,1.0,0.1 mg·kg-1采用免疫组化方法检测海马BDNF和GFAP蛋白的表达。结果大黄酚脂质体可显著提高BDNF的表达,降低GFAP的表达,减轻海马损伤,其中以10.0 mg·kg-1组大黄酚脂质体的作用最为明显(P<0.05)。结论脑缺血再灌注损伤后应用大黄酚脂质体对海马具有明显的保护作用,其机制可能与上调BDNF的表达,降低星形胶质细胞活性有关。
Objective To observe the effect of chrysophanol liposome on the expression of brain-derived neurotrophic factor (BDNF) and glial fibrillary acidic protein (GFAP) after cerebral ischemia-reperfusion injury and to explore the effect of chrysophanol liposome on cerebral ischemia Protective effects and mechanisms of hippocampus in percussion injury mice. Methods The model of cerebral ischemia-reperfusion injury in mice was established by temporarily blocking the common carotid artery. The rats were injected intraperitoneally with 0.1, 0.1 and 0.1 mg · kg-1 chrysophanol liposomes to detect the expression of BDNF and GFAP protein in hippocampus expression. Results Chrysophanol liposome could significantly increase the expression of BDNF, decrease the expression of GFAP and alleviate the damage of hippocampus. The effect of chrysophanol liposome was most obvious (P <0.05). Conclusion The application of chrysophanol liposomes after cerebral ischemia-reperfusion injury has a significant protective effect on hippocampus. The mechanism may be related to up-regulating the expression of BDNF and decreasing the activity of astrocytes.