【摘 要】
：
猪繁殖与呼吸综合征(Porcine reproductive and respiratory syndrome,PRRS)也称猪蓝耳病,是由猪繁殖与呼吸综合征病毒(Porcine reproductive and respiratory syndrome virus,PRRSV)引起的一种严重的病毒性传染病,该病以引起母猪的繁殖障碍、仔猪和育成猪呼吸道症状及高死亡率为主要特征。该病传播速度极快,是
【机 构】
：
浙江理工大学生命科学学院,杭州,310018;浙江省家蚕生物反应器和生物医药重点实验室,杭州,310018
【出 处】
：
中国生物化学与分子生物学会2016年全国学术会议
论文部分内容阅读
猪繁殖与呼吸综合征(Porcine reproductive and respiratory syndrome,PRRS)也称猪蓝耳病,是由猪繁殖与呼吸综合征病毒(Porcine reproductive and respiratory syndrome virus,PRRSV)引起的一种严重的病毒性传染病,该病以引起母猪的繁殖障碍、仔猪和育成猪呼吸道症状及高死亡率为主要特征。该病传播速度极快,是危害我国养猪业的一个重要传染病。疫苗免疫是控制该病发生的一个有效办法,但目前现有疫苗存在许多不足,如PRRSV灭活疫苗免疫剂量大,接种次数多,不能激发黏膜免疫,保护效果不确实；弱毒疫苗虽然可以形成有效的临床保护,但和灭活疫苗一样不能阻止野毒感染,且存在着散毒和毒力返强之忧。所以利用生物技术手段开发能够激发黏膜免疫并能通过血清学方法区分野毒感染的安全和有效的新型PRRS疫苗很有必要。杆状病毒属于昆虫病毒,作为一种高效基因表达载体被广泛用于外源基因的表达研究,该表达系统能够生产具有翻译后修饰的蛋白产物,接近于天然的蛋白结构和生物学活性,杆状病毒载体除具备病毒载体的优势外,还具备安全、制备简单和易于得到高滴度的病毒粒子等优点。本研究中我们拟选PRRSV蛋白M、GP4、GP5的主要抗原表位,融合后在杆状病毒表面展示表达,通过动物免疫接种后研究PRRSV三种囊膜蛋白融合表达产生的免疫效果。
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