【摘 要】
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Recently, nano-HPLC coupled with MS or MS/MS has been widely used in proteome study for the analysis of protein tryptic peptides to achieve high separation
【机 构】
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National Chromatographic R.& A.Center, Dalian Institute of Chemical Physics, Chinese Academy of Scie
【出 处】
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DaLian Internationall Symposia and Exhibition on Chromatogra
论文部分内容阅读
Recently, nano-HPLC coupled with MS or MS/MS has been widely used in proteome study for the analysis of protein tryptic peptides to achieve high separation efficiency and high detection sensitivity.Because of the low flow rate, injectors and transfer tubes with especially small dead volume should be used in such systems to minimize the pre-column volume.Thus, the sample injection volume should be limited in nano liter level.At the meantime, to ensure enough mount of sample injected for MS analysis, laborious off-line concentration steps should be applied, resulting in time consuming and sample losing.
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