The increasingly used real time quantitative reverse transcription-PCR (qRT-PCR) method for gene expression analysis requires one or several reference gene(s) acting as normalization factor(s).In orde
Sugarcane yellow leaf virus (SCYLV) (genus Polerovirus,family Luteoviridae),the causal agent of sug arcane yellow leaf disease (YLD),was first detectedin China in 2006.To assess the distribution of SC
Sugarcane yellow leaf virus (SCYLV; genus Polerovirus,family Luteoviridae) is a recombinant virus associated with yellow leaf disease,a serious threat to sugarcane in China and worldwide.Among the nin
Post-transcriptional gene silencing is commonly observed in polyploid species and often poses a major limitation to plant improvement via biotechnology.Five plant viral suppressors of RNA silencing we
Dirigent and dirigent-like family proteins contain a number of proteins involved in lignification or in the response to pathogen infection and abiotic stress in plants.In the present study,a full-leng
Catalase is an iron porphyrin enzyme,which serves as an efficient scavenger of reactive oxygen species (ROS) to avoid oxidative damage.In sugarcane,the enzymatic activity of catalase in a variety (Yac
甘蔗遗传背景复杂,转基因技术是甘蔗品种遗传改良的重要途径.甘蔗转基因遗传改良主要是通过基因枪法和农杆菌介导法来实现,但是目前我国的甘蔗转基因技术,普遍存在着转基因效率偏低,获得的转基因株系偏少,难以筛选到目的基因稳定表达,有利用价值的转基因株系等问题.本实验室通过多年的经验积累,建立了一种高效的快速的农杆菌介导法的甘蔗转基因方法.转化效率为20％左右,筛选效率达到了90％,转化时间为4个月左右,操