Single and repeated morphine administrations differentially regulate expression of Nethylmaleimide-s

• 作 者: XingLIU;Hao-HongLI;LanMA;
• 机 构: Pharmacology Research Center, Shanghai medical college, institutes of Brain science and state key la
• 出 处: 中国神经科学学会第四次会员代表大会暨第七届全国学术会议(The 7th Biennial Meeting and the
• 发表日期: 2007年期

　　The present study investigated the potential effects of single and repeated morphine treatments on gene expression of N-ethylmaleimide-sensitive protein,a key regulatory protein of neurotransmitter release and synaptic plasticity.After a single morphine injection (10 mg/kg),N-ethylmaleimide-sensitive factor gene expression increased significantly in the cortex,hippocampus and periaqueductal gray.In contrast,N-ethylmaleimide-sensitive factor gene expression was not changed after repeated morphine exposures in these brain regions.Sixteen hours after cessation of repeated morphine admires tration,N-ethylmaleimidesensitive factor mRNA level increased again to a level approximate to that of the single morphine injection group in the hippocampus,peniaqueductal gray and cerebral cortex.These findings suggest that the N-ethylmaleimide-sensitive factor may be involved in the maladaptive changes in the nervous system after opiate treatment.Then we investigate the effects of entorhinal cortex in cue induced cocaine-seeking behavior in rat.We also find that beta-arrestin2 plays an important role in learning and memory of mice.