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Objective: To distinguish three species of Moghania and ensure their safety and efficacy.methods: Three species of M.macrophylla (Willd.)O.Kuntze, M.Latifolia Benth(Moghania) and M.glutiaosa (Prain) Y.T.Wei from Guangxi, Yunnan and Sichuan Province of China(SWUN).Fresh materials were fixed in FAA.Samples were passed through the traditional ethanol and dimethylbenzene series, embedded in paraffin, and then sliced with a microtome and stained with safranine-fast green and finally mounted.The leaf lower epidermal cells are obtained by the practical peeling technique and clearing method with nail polish blot.The materials were dissociated by Nitric acid Chromate method.The samples of herbs were powdered.Results: The three species of Moghania were studied to compare the distinguishing morphoanatomic details of root, stem, leaf and petiole, dissociation and powders.The microscopic features were systematically described and illustrated.And detailed key authentication parameters based on these anatomic characteristics were presented.A key was constructed to the three species for convenient classification.Microscopy can be unambiguously used to authenticate and.distinguish three species of Moghania.