【摘 要】
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OBJECTIVE To evaluate the protective effects of total flavonoid from Litsea Coreana (TFLC) on alcoholic fatty liver (AFL) in rats, and investigate the possible mechanism.METHODS Sixty SD rats were ran
【机 构】
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School of Pharmacy, Anhui Medical University, Hefei 230032, China ; Key Laboratory for Bioactivity o
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OBJECTIVE To evaluate the protective effects of total flavonoid from Litsea Coreana (TFLC) on alcoholic fatty liver (AFL) in rats, and investigate the possible mechanism.METHODS Sixty SD rats were randomly distributed into six groups: normal control (NC), sucrose control (SC), alcohol (model group, M), and alcohol with TFLC (100,200,400 mg·kg-1).AFL model in rats was established by intaking different doses of alcohol (concentration from 5% to 40%) over 12 weeks.Serum levels of TG, TC, LDL-C, HDL-C, TNF-α, insulin, and glucose were measured, histopathologic changes were determined, and expression of adipose differentiation-related protein (ADRP) in the liver were evaluated by Western blotting and immunohistochemistry, respectively.RESULTS Treatment with TFLC resulted in decreased serum levels of TG, TC, LDL-C, TNF-α, glucose and insulin, as well as improved liver index.Morphological evaluation revealed rats in model group developed a severe steatosis, but the severities of liver steatosis were effectively ameliorated in TFLC (200 and 400 mg· kg-1) treated groups.Expression of hepatic ADRP were increased in model group, and suppressed in TFLC treated groups.CONCLUSION TFLC had a protective effect on AFL rats, the mechanism may be involved in regulation serum lipid profiles via down-regulation of hepatic expression of ADRP in AFL rats.
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