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Injection of complete Freunds adjuvant (CFA) to the hind paw of the rat is a widely used animal model of chronic inflammatory pain,after CFA administration,animal will display a hypersensitivity to thermal and mechanic stimuli,which can be characterized by a rapid reduction in paw withdrawal latency to thermal and mechanic stimuli.Recently,spinal astrocytes and microglia have been recognized as potential active participants in the initiation and maintenance of hyperalgesia.Glutamate is one of the predominant excitatory neurotransmitters.Previous studies demonstrated that peripheral group Ⅲ mGluRs play a critical role in the acute inflammatory pain induced by hindpaw administration of Formalin.In the present study,CFA pain model was employed in which observation of nociceptive behavior in response of thermal stimuli,and immunohistochemical examination of activated marker of spinal astrocytes (GFAP) were carried out,combined with peripheral administration of L-SOP,an agonist of group Ⅲ mGluRs,to determine the role of peripheral group Ⅲ mGluRs in the spinal astrocytes activation and long-term hyperalgesia induced by CFA.