【摘 要】
：
Objective Tenovin-6 is a newly reported small molecular inhibitor of Class Ⅲ HDACs or Sir2 family of enzymes.It has been shown to reverse the deacetylation activity of SIRT1, the closest homo1ogues of
【机 构】
：
暨南大学药学院,广州,510632
【出 处】
：
2015医学前沿论坛暨第十四届全国肿瘤药理与化疗学术会议
论文部分内容阅读
Objective Tenovin-6 is a newly reported small molecular inhibitor of Class Ⅲ HDACs or Sir2 family of enzymes.It has been shown to reverse the deacetylation activity of SIRT1, the closest homo1ogues of Sir2 in mammalian which is involved in diverse cellular processes including chromatin remodeling, aging, DNA damage response and tumorigenesis etc.Treatment in pediatric acute lymphoblastic leukemia (ALL) is becoming more and more successful though relapsing is still a problem.In the present study,we studied the effect of Tenovin-6 in pre-B acute lymphoblastic leukemia (ALL) and the underlying mechanisms.
其他文献
目的 缬草是一种败酱科植物,其根和根茎富含挥发油,具有一定的药用价值.我们的前期研究通过对缬草有效成分的分离提取,得到了一种具有抗肿瘤活性的化合物单体Desacetylisovaltratum (DI),且在前期筛选中发现DI对胃癌细胞株的体外抑瘤作用较强.因此,本研究旨在阐明DI对胃癌细胞株的体外增殖抑制作用及相关机制.方法 用MTT法检测胃癌细胞株增殖.用流式细胞术检测细胞凋亡与周期分布.用w
药物治疗是恶性肿瘤治疗的重要手段之一,自噬是依赖溶酶体途径对胞质蛋白和细胞器进行降解的一种过程,在肿瘤的发生发展及治疗中起重要作用。为了阐明抗肿瘤药物能否激活自噬及其分子机制以及自噬对药物疗效影响等,我们进行了系列深入研究,结果发现DNA损伤类抗肿瘤药物通过激活SAPK/JNK通路转录上调Beclin1,LC3,Sestrin2等自噬相关基因的表达以及通过ATM磷酸化PTEN促进其转位入胞核激活J
目的 阐明miR-181a-5p对巨噬细胞表型转化的作用,并初步探讨其作用机制。方法 培养人白血病单核细胞系THP-1,用PMA将其诱导为静息态的巨噬细胞(Mφ),然后加入LPS和IFN-γ诱导为M1型巨噬细胞或加入IL-4诱导为M2型巨噬细胞；通过Real-time PCR和Western blot方法检测不同表型巨噬细胞中M1型和M2型特异性标志物CD163等mRNA及蛋白表达水平；采用ELI
The aim of the present study was to investigate how the miR-451a can regulate resistance to tamoxifen (TAM) of breast cancer cells by modulating the expression of estrogen receptor α (ERα) and 14-3-3ζ
NEK8 (Never-in-Mitosis A-related kinase 8)为人类NIMA相关丝/苏氨酸蛋白激酶家族成员,参与细胞骨架、原纤毛结构维持及DNA损伤应答/修复等过程调控.NEK8表达或功能异常与肿瘤等疾病发生密切相关,但其自身表达调控机制尚待阐明.本文揭示缺氧可诱导肿瘤细胞nek8基因表达上调,大鼠肾脏缺血再灌注损伤模型在整体水平也证实缺氧可上调NEK8蛋白表达；而且证实ne
Signal transducer and activator of transcription 3 (STAT3) is constitutively activated in malignant tumors and plays important roles in multiple aspects of cancer aggressiveness.Thus, targeting STAT3
Purpose To illustrate the mechanism of IAPs sustain the properties of NPC cancer stem-like cells and identificate the potent of targeting IAPs in the therapy of NPC.Method FACS was used to sort SP cel
目的 阐明miR-100抑制乳腺癌细胞侵袭转移的作用与机制。方法 qRT-PCR实验比较分析了10例癌旁和69例乳腺癌组织,及正常乳腺上皮细胞MCF-10A和MCF-7等6株不同乳腺癌细胞的miR-100表达；MDA-MB-231和MCF-7细胞中分别转染miR-100 mimic和miR-100 inhibitor后,transwell侵袭实验分析miR-100对乳腺癌细胞侵袭能力的影响；荧光素
Background: T-type Ca2+ channels are often aberrantly expressed in different human cancers and participate in the regulation of cell cycle progression, proliferation and death.Methods: RT-PCR, Q-PCR,
目的 检测灭绦灵对Bcr Ab1-T315I细胞的杀伤作用,以明确其克服imatinib耐受性Bcr Ab1-T315I突变引起的耐药.方法 慢性粒细胞性白血病(CML)细胞KBM5(imatinib敏感)和KBM5-T315I(imatinib耐受)细胞经梯度稀释的药物处理72小时,用MTS法检测灭绦灵抑制增殖的能力；CML细胞经2.0 μmo1/L灭绦灵处理48小时后,用PBS洗去残存的药物,