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A moderate halophilic PAH-degrading strain Martelella sp. AD-3 was developed from highly saline petroleum-contaminated soil by enrichment on anthracene as the sole carbon and energy source. The experiments were conducted to evaluate PAHs biodegradation capacity of halophilic strain AD-3 and propose its possible phenanthrene metabolic pathway. Halophilic strain AD-3 was shown to grow on phenanthrene as sole carbon and energy source efficiently under multiple conditions characterized by different concentrations (100 to 400 mg·L-1), broad salinities (0.1 to 15%) and varying pHs (6.0 to 10.0).