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超氧化物歧化酶(Superoxide Dismutase简称SOD)因能清除超氧阴离子而与机体的衰老、肿瘤、免疫性疾病和辐射防护等有关,SOD的活性测定也开始应用于临床生化及药物监测。 SOD的测活方法很多,常见的有化学法、免疫法和等电点聚焦法。以化学法应用最普遍。包括黄嘌呤氧化酶法、连苯三酚法氮兰四唑法和化学发光法等。但其仪器要求高、操作方法较繁琐、试剂昂贵且来源困难,给临床开展SOD的活性测定带来困难。本文就连苯三酚法在临床应用上作了一些探讨。
Superoxide dismutase (SOD), which can remove superoxide anion, is related to the body’s aging, tumor, immune disease and radiation protection. The activity determination of SOD also began to be used in clinical biochemistry and drug monitoring. Survival of SOD many ways, there are common chemical methods, immunization and isoelectric focusing method. The most common use of chemical methods. Including xanthine oxidase method, pyrogallol method nitrogen blue tetrazolium method and chemiluminescence and so on. However, the high requirements of the instrument, the method of operation is more cumbersome, the reagents are expensive and the sources are difficult, which makes it difficult to carry out the activity determination of SOD in clinic. This article on the pyrogallol method in clinical application made some discussion.