【摘 要】
：
The maturing sperm cells discard the majority of their cytoplasm during the final stages of spermatogenesis and lose some of their defense-enzymes.The purpose of the present study was to investigate t
【机 构】
：
College of Animal Science and Technology, Northwest A & F University, Yangling,Shaanxi 712100, P.R.C
【出 处】
：
2011中国肉牛选育改良与产业发展国际研讨会
论文部分内容阅读
The maturing sperm cells discard the majority of their cytoplasm during the final stages of spermatogenesis and lose some of their defense-enzymes.The purpose of the present study was to investigate the effects of Vitamin E supplementation on standard semen quality parameters and anti-oxidant activities of frozen-thawed bovine sperm.Vitamin E was added at concentrations of 0.5,1.0, 1.5 and 2.0 mg/ml to bovine semen cryoprotective medium.The results showed that the sperm motility and VSL, STR values in the extender supplemented with 1.0 and 1.5 mg/ml Vitamin E were significantly higher than that of other concentrations (p<0.05).The percentages of acrosome-intact and membrane-intact sperm were significantly improved (p<0.05) by supplementing with 1.5 mg/ml Vitamin E.In biochemical assays, the extender supplemented with Vitamin E did not exhibit significant improvement in SOD levels, compared with the control (p>0.05).Compared with the other groups, CAT levels were demonstrated to be greater with the supplementation of Vitamin E at 1.0 and1.5 mg/ml (P<0.05).The extender supplemented with 1.5 mg/ml Vitamin E caused the highest levels of GSH-Px, compared with other groups (p<0.05).The GSH activity was significantly higher with the supplementation of 0.5, 1.0 and 1.5 mg/ml Vitamin E, compared with 2.0 mg/ml Vitamin E group and control (p<0.05).Moreover, increasing the doses of Vitamin E decreased sperm antioxidant activities,the extender supplemented with 2.0 mg/ml Vitamin E caused the lowest levels of GSH-Px and GSH activities, compared with other treatment groups (p<0.05).In conclusion, the beneficial effects of Vitamin E noted in the present study can be attributed to the antioxidant characteristics.Vitamin E supplementation in the extender reduced the lipid peroxidation potential and improved semen quality during the freezing-thawing.More researches are needed to evaluate and understand the precise physiological role of Vitamin E in reproduction.
其他文献
Somatostatins play a crucial role in the regulation of growth and development in vertebrates,especially muscle growth.We assessed the association of somatostatin gene polymorphisms with growth traits
In this study, using PCR-SSCP and DNA sequencing technology, the association of single nucleotide polymorphisms (SNP) in the bovine MyoG gene with body measurement traits was first assessed in 779 ind
Acyi-coenzyme A oxidase 1 (ACOX1) is the first and rate-limiting enzyme in peroxisomal fatty acid β-oxidation and plays a key role in fatty acid metabolism and fat deposition.It is an important candid
Myopalladin (MYPN) is a multifunctional protein whose main role is to maintain sarcomeric integrity and regulate Z-line structure.It is an important candidate gene for meat quality selection through m
The fatty-acid desaturase enzyme could convert n-6 polyunsaturated fatty acids into ω-3polyunsaturated fatty acids (ω-3PUFAs), which found mainly in fish Oils, recognized as essential in preventing ma
To improve the freezing efficiency and avoid the contamination of liquid nitrogen in early bovine embryos, this study developed a vitrification method--the closed pulled straw (CPS), which has the adv
Egg low-density lipoprotein (LDL) was added at concentrations (w/v) of 7%, 8% or 9% to the extenders used to freeze bull semen and its effects on seminal parameters and antioxidant activities of froze
This study measured the hypothesis that Rhodiola Sachalinensis Saccharide (RSS) had the positive effect on bovine cryopreservation sperm.RSS was extracted from rhodiola sachalinensis (R.sachalinensis)
Agriculture Environmental Renewal Canada (AERC) Inc.is a Canadian research and development seed company specialized in the development of forage, silage and grain hybrids of sorghum (Sorghum bicolor,
Canadian Forage Sorghum Hybrid 30 (CFSH 30) produces better yields with excellent forage quality over a wide range of growing conditions.This hybrid has been tested widely across several locations in