论文部分内容阅读
Introduction: Metals contamination especially in agricultural soils is the burning issue in the present era.Although,some heavy metals are essential for plant growth but their excessive concentration in soil can result in toxicity.And these heavy metals(V,Cd,Cu,Fe Pb,Zn etc.)toxicity affects the growth,morphology and also causes biochemical changes in plants by different ways.Vanadium(V)is widely distributed in earth crust however; its bioavailability to plants mainly depends upon parent material and characteristics of the soil.Purpose: The present work was designed: 1.To evaluate the growth response of chickpea plants to V stress and its accumulation.2.To determine the effect of V stress on DNA.Methodology: Chickpea seeds were sown in the polythene bags containing artificially contaminated red soil(400 g/bag)in controlled environment.Ammonium metavanadate(NH4VO3)was used to contaminate the soil.There was six treatments applied(T1= control,T2=50 mg V/kg,T3=90 mg V/kg,T4=130 mg V/kg,T5=170 mg V/kg,T6=200 mg V/kg)according to complete randomized design with five replications.The distill water is using to maintain the moisture content.After 30 days of sowing,the plants will be harvested and samples will be collected.The following parameters will be analyzed to assess the effect of V on chickpea plants grown in red soil; height of plants,plant biomass,and root: shoot ratio of V,tolerance index,bioaccumulation factor,accumulation of V in roots and shoots,DNA degradation and electrolyte leakage.Results: The results will be summarized after the harvesting(till end of June,2014)and will be presented in the 17th International Conference on Heavy metals in the Environment(ICHMET)held at Guiyang,China.Conclusion: The findings will be supportive to evaluate the effects of V on chickpea plants and will be used as guidance for soil health especially for red soils and also will be helpful for further study.