【摘 要】
：
To understand biology at system level, examining the structure and dynamic changes under different status can gain more insights than characterizing isolated pathways of cells or organisms.Rheumatoid
【机 构】
：
National ChromatograPhic R.& A.Center, Dalian Institute of Chemical Physics, Chinese Academy of Scie
【出 处】
：
DaLian Internationall Symposia and Exhibition on Chromatogra
论文部分内容阅读
To understand biology at system level, examining the structure and dynamic changes under different status can gain more insights than characterizing isolated pathways of cells or organisms.Rheumatoid arthritis (RA), a disease involved multiple organ and system disorders, is still attractive to many researchers and leaves much enigma to be cracked.In this study, we demonstrated the application of the combined genomics and metabonomics strategy to explore the major metabolic traits of RA patients.Peripheral blood mononuclear cells (PBMCs) were isolated from normal controls and the patients.mRNA expression analysis showed 147 differentially expressed genes nearly involved in all biological processes after retrieved through KEGG, Biocarta, SwissPort and Gene Ontology: Biological Processes databases.Sera samples were then subjected to metabonomics assay utilizing ultra performance liquid chromatography-time of flight mass spectrometry.
其他文献
Three micron hydrophilic interaction liquid chromatography (HILIC) columns, TSKgel Amide-80 3μm were developed for use in glycomic stydies or in LC/MS applications for hydrophilic compounds including
Numerous chemical compounds can form persistent toxic residues in air, soil, water and foods.Pesticides are one of the principal classes of such environmental pollutants which results in serious safet
Three micron ODS columns, TSKgel ODS-100V 3μm were developed for use in LC/MS applications, in particular for the analysis of active pharmaceutical ingredients and their metabolites.The behavior of th
Advanced glycation end products (AGEs) are a heterogeneous group of proteins that have been modified with glucose or carbohydrates adducts, and are thought to be responsible for many complications of
Aqueous size exclusion chromatography is a powerful tool for determining of bio polymers like peptides, proteins and DNAs.TSK-GEL SW series is one of typical aqueous SEC columns for analyzing biologic
A fast and simple method, which involves continuous-flow microextraction (CFME) followed by high-performance liquid chromatography (HPLC) and variable-wavelength detection (VWD), has been developed to
In this paper, a new stationary phase based on 1-allyl,3-methylimidazolium chloride ([AMIM]Cl)ionic liquid were synthesized by vinyltriethoxysilane modified silica and [AMIM]Cl.The capacity factors (k
Superose 12 column, which is filled with the 12% cross-linked agarose gel, is often used to separate and purify high molecular weight compounds.The application of this matrix for isolation and purific
Hyphenated techniques such as GC or LC combined with mass spectrometry provide a unique combination of separation and detection power.With its ease-of-use and lower cost, GC/MS and LC/MS with single q
The rapidly growing interest in the area of proteomics induces intensive efforts to find robust, automated and sensitive high-throughput analytical tools.One of the key advancements in rapid protein i