Dysfunction of human adipose tissue-derived stem cells induced by advanced glycation end products an

来源 :第五届国际络病学大会 | 被引量 : 0次 | 上传用户:YAOXUEQIN
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Objective To study the effects of advanced glycation end products-human serum albumin(AGEHSA) on the oxidative stress,proliferation,migration and serection of adipose tissue derived stem cells(ADSCs),and the involved molecular mechanisms.Methods The subcutaneous adipose tissue samples were obtained from female patients receiving cosmetic surgery,then enzymatically digested and centrifugated in vitro.The ADSCs were determined by fluorescence activated cell sorter(FACS) analysis and in vitro differentiation induced according to different previous strategies.After stimulated with different dosages of AGE-HSA,the expression of RAGE,MAPK and NF-κB was determined by western blot.
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