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Thermosensitive self-assembling hydrogels displaying lower critical solution temperature (LCST) behavior are liquid at room temperature and solidify at body temperature.Given the mild crosslinking mechanism,these gels are very suitable for encapsulation of living cells for regenerative medicine applications.To stabilize these physically crosslinked gels,covalent cross-linking by photopolymerization is often applied.Besides photopolymerization,we use also morebiofriendlycrosslinking methods such as oxo-ester mediated native chemical ligation (OMNCL).