Deep-degree burned dermal matrix(DDBDM) may become a ideal dermal graft compared with acellular derm

• 作 者: GUANYING YU LAN YE XUGUO ZHU YAONAN LI DUYIN JIANG
• 机 构: Department of Burn,The Second Hospital of Shandong University,Jinan,Shandong 250033;Jinan Central Ho
• 出 处: 第十届全国创伤修复(愈合)与组织再生学术会议、第三届中欧创面修复学术会议、中国医师协会创伤外科医师分会创面修复医师专业委
• 发表日期: 2016年4期
• 关键词: acellular dermal matrix deep-degree burned dermal matrix deep burn cytokine derm

　　Background: Second and third degree burns present a broad range of additional challenges for restoration of function.The extensive skin defect induced by severe burns is dangerous and fatal to burned patients.The common therapy method used to treat the extensive burns is the dermatoplasty following tangential excision to remove the necrotic or denatured skins.But the source of transplanting skins is often limited.The xenogenic dermal substitutes,such as porcine acellular dermal matrix(ADM),was used to cover the burn wounds,which could prevent infection,aid to skin renewal and vascularization,accelerate wound healing.We assumed that the burned skins with partly biological activity might be recycled to make the acellular dermal matrix,called "deep-degree burned dermal matrix(DDBDM)",used for treating deep burned wounds,which can avoid the histoincompatibility instead of the foreign or xenogenic demal matrix,reduce the therapy costs by making full use of the discarded skins.The successful clinical application of DDBDM will bring a new better therapy method to the burned patients.Method: Kunming mouse were used to build the burn animal models.The trypsin digestion and shock-elution method was used successfully to make the proper dermal matrix.To investigating whether the DDBDM made from burned skins contained the less burn toxin and the beneficial factors,the 308 cytokines were analyzed.To characterize the inflammatory response,the dermal matrix was implanted subcutaneously in healthy Kunming mouse.At weeks 1,2,3 and 4,the tissue specimens were harvested for gross and histologic observation.