论文部分内容阅读
Of the five viroids known to infect grapevine, Grapevine yellow speckle viroid 1 (GYSVd-1) occurs in all Australian commercial wine grape varieties (Chardonnay, Shiraz, Cabernet and Merlot).While methods for the extraction of DNA from wine are known, none has been developed for RNA.To test whether viroid RNA can pass through the fermentation process we developed a rapid protocol for the extraction of total nucleic acids from wines using a silica-based matrix.Both DNA (23 s rRNA gene) and viroid RNA were detected in extracts from wine samples.