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Curcuma longa (Fam.Zingiberaceae) is revered as the golden spice in Traditional Chinese Medicine (TCM) and Ayurveda for its therapeutic benefits and culinary uses.The objective of present research was to carry out the microscopic identification and comparison of tissue specific metabolite distribution in Curcuma rhizomes used in India and China.Laser micro dissection was used for microscopic identification and isolation of cork, cortex and vascular bundles.Metabolite profiling was carried out by UHPLC-QTOF-MStechnique.A database of 89 phytoconstituents was prepared to identity metabolites by LC-MS based upon their molecular characteristics.The results indicate that,the rhizomes of Curcuma collected from China and India had a similar metabolite distribution profile in whole drug samples.In tissue analysis, cortex and cork tissues revealed a higher distribution of phytoconstituents like cyclocurcumin, demethoxycurcumin, and bisacurone compared to the vascular bundles.