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Objective: Four medicinal plants, Taraxacum mongolicum, Viola yedoensis Makino, Rhizoma coptidis, and Radix Isatidis(MYCI), were formulated and the antiviral effect of MYCI formula to porcine epidemic diarrhea(PED) virus was assessed in in vivo experiment.Methods: A total of 22 three-day-old suckling piglets were obtained from commercial farm that was free from PED virus vaccination.The piglets were randomly assigned to four experimental groups(A-D) and housed separately.The piglets in group A were administered MYCI formula one day after challenging with virulent PED virus.Piglets in group B were treated with MYCI formula without challenging with virulence PED.Piglets in group C were challenged by PED virus without treating MYCI formula.Group D was a control group with neither virus challenging nor the administration of MYCI formula.Antiviral effects of MYCI formula were measured by daily weight gain, clinical signs including fecal score, mortality, the detection of virus from feces and small intestine using RT-PCR, and examination of gross lesion and histopathology.Results: Piglets, which were challenged by virulence PED virus followed by treating with MYCI formula, showed significant higher growth performance(P<0.05), no mortality, lower virus she dding in the feces, lower virus detection in the small intestine(P<0.05), milder gross lesion, and higher ratio of villus height to crypt depth than piglets challenged by virulence PED virus without treating with MYCI formula(P<0.05).Conclusion: In conclusion, the MYCI formula manifested antiviral effect on PED virus in piglets.PED virus is a member of the genus corona virus, thus MYCI formula may have a potential therapeutic or prophylactic value for other corona viruses.
Objective: Four medicinal plants, Taraxacum mongolicum, Viola yedoensis Makino, Rhizoma coptidis, and Radix Isatidis (MYCI), were formulated and the antiviral effect of MYCI formula to porcine epidemic diarrhea (PED) virus was assessed in vivo. Methods: A total of 22 three-day-old suckling piglets were obtained from commercial farm that was free from PED virus vaccination. The piglets were randomly assigned to four experimental groups (AD) and housed separately. The piglets in group A were administered MYCI formula one day after challenging with virulent PED virus. Piglets in group B were treated with MYCI formula without challenging with virulence PED. Piglets in group C were challenged by PED virus without treating MYCI formula. Group D was a control group with neither virus challenging nor the administration of MYCI formula. Antiviral effects of MYCI formula were measured by daily weight gain, clinical signs including fecal score, mortality, the detection of virus from feces an d small intestine using RT-PCR, and examination of gross lesion and histopathology. Results: Piglets, which were challenged by virulence PED virus followed by treating with MYCI formula, showed significant higher growth performance (P <0.05), no mortality, lower virus she dding in the feces, lower virus detection in the small intestine (P <0.05), milder gross lesion, and higher ratio of villus height to crypt depth than piglets challenged by virulence PED virus without treating with MYCI formula (P <0.05). Conclusion: In conclusion, the MYCI formula shows antiviral effect on PED virus in piglets. PED virus is a member of the genus corona virus, thus MYCI formula may have a potential therapeutic or prophylactic value for other corona viruses.