PslG, a Self-produced Glycosyl Hydrolase Triggers Biofilm Disassembly by Disrupting Exopolysaccharid

• 作 者: S.Yu T.Su H.Wu S.Liu D.Wang T.Zhao Z.Jin W.Du M.Zhu S.Chua L.Yang D.Zhu L.Gu L.Ma
• 机 构: InstituteofMicrobiology,ChineseAcademyofSciences,Beijing100101,China;UniversityoftheChineseAcademyof
• 出 处: 中国生物工程学会第六次全国会员代表大会暨第九届学术年会
• 发表日期: 2015年11期

Biofilms are surface-associated communities of microorganism embedded in extracellular matrix.Exopolysaccharide is a critical component in the extracellular matrix that maintains biofilm architecture and protects resident biofilm bacteria from antimicrobials and host immune attack.However,the self-produced factors that target the matrix exopolysaccharides,are poorly understood.Here we show that PslG,a protein involved in the synthesis of a key biofilm matrix exopolysaccharide Psl in Pseudomonas aeruginosa,prevents biofilm formation and disassembles existing biofilms within minutes at nanomolar concentrations while supplied exogenously.The crystal structure of PslG indicates the typical features of an endoglycosidase.PslG disrupts mainly the Psl matrix to disperse bacteria from biofilms.PslG treatment markedly enhances biofilm sensitivity to antibiotics and macrophage cells,resulting in improved biofilm clearance in a mouse implant infection model.Furthermore,PslG shows biofilm inhibition and disassembly activity against a wide range of Pseudomonas species,indicating its great potential in combating biofilm-related complications.